American Society of Hematology

Figure 3

$Figure 3. Activation of T cells induces the externalization of FPRL-1 and Anx-A1. (A) FACS analysis of FPRL-1 expression in human peripheral blood T cells incubated with medium alone (white) or stimulated with the indicated concentrations of anti-CD3/CD28 (gray). Results are representative of 3 separate experiments. (B) FACS analysis of FPRL-1 expression over time in unstimulated (white) or anti-CD3/CD28 (2.0 μg/mL)–stimulated (gray) human peripheral blood T cells. Results are representative of 3 separate experiments. (C) Western blot analysis of Anx-A1 levels in the cytosolic (Cyt) and membrane (Mem) fractions of human peripheral blood T cells stimulated with anti-CD3/CD28 (1.0 μg/mL) for 30 minutes. (D) Immunoprecipitation and immunoblotting analysis of Anx-A1 levels in the culture supernatants of human peripheral blood T cells stimulated with and without anti-CD3/CD28 (1.0 μg/mL) for 1 or 3 hours. (E, F) Western blot analysis of total and phospho Erk and Akt of human peripheral blood T cells stimulated with anti-CD3/CD28 (1.0 μg/mL) in the presence or absence of hrAnx-A1 (300 nM) for the indicated time. In all the experiments, results are representative of 3 separate experiments with similar results.$

Activation of T cells induces the externalization of FPRL-1 and Anx-A1. (A) FACS analysis of FPRL-1 expression in human peripheral blood T cells incubated with medium alone (white) or stimulated with the indicated concentrations of anti-CD3/CD28 (gray). Results are representative of 3 separate experiments. (B) FACS analysis of FPRL-1 expression over time in unstimulated (white) or anti-CD3/CD28 (2.0 μg/mL)–stimulated (gray) human peripheral blood T cells. Results are representative of 3 separate experiments. (C) Western blot analysis of Anx-A1 levels in the cytosolic (Cyt) and membrane (Mem) fractions of human peripheral blood T cells stimulated with anti-CD3/CD28 (1.0 μg/mL) for 30 minutes. (D) Immunoprecipitation and immunoblotting analysis of Anx-A1 levels in the culture supernatants of human peripheral blood T cells stimulated with and without anti-CD3/CD28 (1.0 μg/mL) for 1 or 3 hours. (E, F) Western blot analysis of total and phospho Erk and Akt of human peripheral blood T cells stimulated with anti-CD3/CD28 (1.0 μg/mL) in the presence or absence of hrAnx-A1 (300 nM) for the indicated time. In all the experiments, results are representative of 3 separate experiments with similar results.

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