LBW242 decreases survival in Bcl-2–overexpressing MM.1S cells. (A) Empty vector– or Bcl-2–transfected MM.1S cells were treated for 72 hours with LBW242 at the indicated concentrations and then analyzed for viability. Results are means ± SD of 2 independent experiments. Inset shows Western blot of Bcl-2 protein levels. (B) Vector- or Bcl-2–transfected MM.1S cells were treated for 48 hours with LBW242 at the indicated concentrations, and analyzed for apoptosis by DNA fragmentation assays (mean ± SD; n = 2). The percentage of specific cell death was calculated by subtracting the percentage of spontaneous apoptosis of the relevant controls from the total percentage of cell death.