Real-time PCR verification of microarray data. The PCR data were normalized to the mean of 3 housekeeping genes: glyceraldehyde-3-phosphat dehydrogenase (GAPDH), ribosomal protein L13a (RPL), β₂-microglobulin (B2M). Subsequently, the relative N-fold regulation and SEM compared with unstimulated monocytes (n = 3) was calculated (▒) and compared with the results obtained from the microarray analysis (n = 4) (▪). The genes analyzed were interleukin-1 receptor type II (IL1-R2), the immunoglobulin domain containing protein Z39Ig; sin3A-associated protein (SAP30); formyl peptide receptor 1 (FPR); high mobility group box 2 (HMGB2); lymphotoxin beta (LtB); the chemokine receptor CX3CR1; the chemokines CXCL10, CXCL9, and CCL5; the cell-surface protein CD36; and the interleukin 21 receptor (IL21R). Shown are the mean and standard error of the mean (SEM) of 4 individual experiments.