American Society of Hematology

Figure 1

$Figure 1. Selective accumulation of FOXP3+CD4 T cells in tumors compared with PBLs. Cryopreserved tumor digests from 3 patients with metastatic melanoma (Pt) and PBLs from 3 healthy donors (HD) were thawed and immediately stained with CD3, CD8, and FOXP3 mAbs. (A) The dotplots were gated on CD3+ lymphocytes. The quadrants were set based on the isotype control Abs. The percentage for each quadrant represents the fraction of FOXP3+ T cells in CD8 T cells (top right quadrant) or in CD4 T cells (top left quadrant). CD3+CD8− T cells were considered CD4 T cells throughout the study. (B) The percentage of FOXP3+CD4 T cells per total CD4 T-cell population is quantified in PBLs from healthy donors (n = 12) and from patients with melanoma (n = 24) and in tumor digests (n = 26) using FACS analyses. (C) The percentage of FOXP3+CD4 T cells per total CD4 T cells in PBLs and tumors from the same patients (n = 13) were enumerated as described earlier.$

Selective accumulation of FOXP3⁺CD4 T cells in tumors compared with PBLs. Cryopreserved tumor digests from 3 patients with metastatic melanoma (Pt) and PBLs from 3 healthy donors (HD) were thawed and immediately stained with CD3, CD8, and FOXP3 mAbs. (A) The dotplots were gated on CD3⁺ lymphocytes. The quadrants were set based on the isotype control Abs. The percentage for each quadrant represents the fraction of FOXP3⁺ T cells in CD8 T cells (top right quadrant) or in CD4 T cells (top left quadrant). CD3⁺CD8⁻ T cells were considered CD4 T cells throughout the study. (B) The percentage of FOXP3⁺CD4 T cells per total CD4 T-cell population is quantified in PBLs from healthy donors (n = 12) and from patients with melanoma (n = 24) and in tumor digests (n = 26) using FACS analyses. (C) The percentage of FOXP3⁺CD4 T cells per total CD4 T cells in PBLs and tumors from the same patients (n = 13) were enumerated as described earlier.

This Feature Is Available To Subscribers Only