The procedure for isolating spleen cDC subtypes. A cDC-enriched preparation was prepared by either the standard procedure, or with an additional immunomagnetic particle depletion step to eliminate cells bearing NK cell markers. Final purification by flow cytometric cell sorting involved selection for CD11c^hi cDCs then sorting into CD4⁺8⁻, CD4⁻8⁻ or CD4⁻8⁺ subtypes. Full details are in “Materials and methods.”