Subpopulations within pDC preparations. (A) The procedure for isolating pDCs and IKDCs. The initial steps were similar to those used for cDCs (Figure 1) except the immunomagnetic bead depletion mAb cocktail differed. No depletion of cells bearing NK cell markers was used. Rather, the pDCs were isolated by flow cytometric cell sorting (CD45RA^hi CD11c^int) and segregated into Ly6C⁺ and Ly6C⁻ subsets, and the IKDCs were segregated as CD49b⁺ and CD11c^int cells, either CD45RA^hi or CD45RA^int. Full details are in “Materials and methods.” (B) The correlation between Ly6C and Ly49Q expression by pDC preparations. pDCs were isolated as in panel A and gated on sorting as CD11c^int CD45RA^hi cells.