Common pathway gene expression patterns in leukemic and nonleukemic hematopoietic tissues. (A) qRT-PCR analysis of Fos, Jun, Egr-1, Tnf, and Vcam1 expression levels was performed using primers specific for each cDNA. All data were normalized to Gapdh, since the expression of this gene does not change during myeloid differentiation (Figure 5C). Day-2 samples were obtained from 2 independent myeloid differentiation experiments. The 6 APL samples used were the same as those used in the studies shown in Figures 4–5. All RNA samples used for this study were nonamplified. (B-F) Common pathway gene expression patterns in whole bone marrow and spleen samples using nonamplified RNA- and array-based expression profiling. Two grouping variable category graphs show raw probe set signal intensity values for the common pathway genes identified in Figure 5. Unmanipulated bone marrow and spleen samples were obtained from either wild-type mice (WT), or from mCG-PML-RARα mice (KI), and were cryopreserved prior to analysis. APL samples were obtained from the cryopreserved spleens of 18 overtly leukemic mCG-PML-RARα mice, including the 6 APL samples in Figures 3C, 4, and 5 (Table S2, APL samples 1-18).