Ectopic RXRα impairs the induction of LF⁺ granulocytes. Human CD34⁺ cells were transduced with vectors encoding RXRα, RXRαΔ, or empty control vector (CTRL). After transduction (48 hours), cells were subcultured in serum-free G-specific cultures in the absence (vehicle) or presence of NR ligands (100 nM) as indicated. Generated cells were analyzed at day 12 by FACS for GFP versus intracellular LF and cell-surface CD14. FACS diagrams represent gated GFP⁺ cells analyzed for LF versus CD14. Data are representative of 6 independent experiments. Bars represent the mean and SD of the percentage of LF⁺ or the percentage of LF⁻CD14⁺ cells among gated GFP⁺ cells of 6 independent experiments. *Significant differences at P < .05 according to a general linear statistical model; ns indicates not significant.