Ectopic RXRα augments monocyte features. (A) Human CD34⁺ progenitor cells were transduced with vectors encoding RXRα, RXRαΔ, or empty control (CTRL) under progenitor expansion conditions. After transduction (48 hours), cells were subcultured in serum-free G-specific cultures in the absence (veh) or presence of LG100268 (100 nM) or VD3 (60 nM) as indicated. Generated cells were analyzed by FACS for GFP versus CD14 and CD115 (M-CSFR). Bars represent the mean percentage and SD of the percentage of CD14⁺M-CSFR⁺ cells among gated GFP⁺ cells observed in 4 independent experiments. (B-C) CD34⁺ cells were transduced with empty control (CTRL) RXRα- or RXRαΔ-encoding vectors under progenitor expansion conditions. After transduction (48 hours), cells were replated in serum-free Mo-specific cultures in the absence (vehicle) or presence of LG100268 or VD3 as indicated. GFP⁺ cells were analyzed by FACS for CD11b versus CD14. (B) One representative of 5 independent experiments is shown (P < .05 for the percentage of CD11b⁺CD14⁺). Bars in panel C represent the average CD11b mean fluorescence intensity and SD calculated from 5 independent experiments. (D) HL60e cells transduced with empty control vector, RXRα, or RXRαΔ. Cells were cultured for 48 hours in the absence or presence of LG100268 or VD3 as indicated. GFP⁺ cells were gated and analyzed for CD11b. Bars represent the mean and SD calculated from 3 independent experiments. (A-D) *Significant differences at P < .05 according to a general linear statistical model; ns indicates not significant.