American Society of Hematology

Figure 4

$Figure 4. Altered polysome profiles in TF-1 cells depleted of RPS19. Cells extracts were prepared for polysome analysis as described in “Materials and methods.” TF-1 cells infected with a lentivirus containing siRNA A targeted to RPS19 were grown for 4 days in the presence (+DOX) or absence (−DOX) of 0.5 μg/mL DOX. Extracts were layered on 15% to 55% sucrose gradients, and centrifugation was carried out for 5 hours at 67 000g. Gradients were fractionated using an ISCO-type 185 gradient fractionator, and absorbance at 254nm was monitored with a UA-6 absorbance detector.$

Altered polysome profiles in TF-1 cells depleted of RPS19. Cells extracts were prepared for polysome analysis as described in “Materials and methods.” TF-1 cells infected with a lentivirus containing siRNA A targeted to RPS19 were grown for 4 days in the presence (+DOX) or absence (−DOX) of 0.5 μg/mL DOX. Extracts were layered on 15% to 55% sucrose gradients, and centrifugation was carried out for 5 hours at 67 000g. Gradients were fractionated using an ISCO-type 185 gradient fractionator, and absorbance at 254_nm was monitored with a UA-6 absorbance detector.

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