Figure 2
Figure 2. Erythrocyte abnormalities and peripheral lymphopenia in Gimap5−/− mice. (A) Peripheral blood smear (Wright-Giemsa stain, original magnification ×400). Bars represent 10 μm. wt indicates wild-type; −/−, Gimap5-knockout. Images were acquired on a Nikon Eclipse E600 microscope with 10×, 20×, and 40× PlanFluor objectives and a SPOT Insight 11.2 color mosaic digital camera with Spot software version 4.1 (Diagnostic Instruments, Sterling Heights, MI). (B) Representative FACS analysis of wild-type (wt) and Gimap5-deficient (−/−) splenocytes and inguinal lymph node cells (INL) for expression of CD8 and CD4. Boxes indicate gates used to calculate frequencies presented in Table 1. Gimap5−/− mice exhibit peripheral lymphopenia, with reduced abundance of CD4 T cells and almost complete absence of CD8 T cells from the spleen and inguinal lymph nodes (INL).

Erythrocyte abnormalities and peripheral lymphopenia in Gimap5−/− mice. (A) Peripheral blood smear (Wright-Giemsa stain, original magnification ×400). Bars represent 10 μm. wt indicates wild-type; −/−, Gimap5-knockout. Images were acquired on a Nikon Eclipse E600 microscope with 10×, 20×, and 40× PlanFluor objectives and a SPOT Insight 11.2 color mosaic digital camera with Spot software version 4.1 (Diagnostic Instruments, Sterling Heights, MI). (B) Representative FACS analysis of wild-type (wt) and Gimap5-deficient (−/−) splenocytes and inguinal lymph node cells (INL) for expression of CD8 and CD4. Boxes indicate gates used to calculate frequencies presented in Table 1. Gimap5−/− mice exhibit peripheral lymphopenia, with reduced abundance of CD4 T cells and almost complete absence of CD8 T cells from the spleen and inguinal lymph nodes (INL).

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