In vivo repopulation advantage of FA-D1 BM cells transduced with BRCA2-LVs in the PB of FA-D1 recipients conditioned with mild irradiation. (A) Influence of a mild conditioning of 3 Gy to facilitate the engraftment of FA-D1 recipients with moderate numbers (500 000 Lin⁻ cells) of fresh BM cells from WT mice. Follow-up of donor engraftments obtained between 1 and 3 months after transplantation. □ and ■ represent unirradiated recipients and recipients irradiated with 3 Gy before the BM transplantation, respectively. (B) Kinetics of donor hematopoietic repopulation in FA-D1 recipients conditioned with 3 Gy and transplanted with 500 000 FA-D1 Lin⁻ BM cells that were transduced with BRCA2- or EGFP-LVs. Donor repopulation data were obtained by qPCR analyses of SRY sequences (Y-chromosome specific) in PB samples from recipient mice. Mice 1 to 6 were transplanted with BRCA2-transduced cells. Mice 7 to 9 were transplanted with EGFP-transduced cells.