Kinetic profile of LV content within the liver and spleen following systemic injection. (A) Quantification of LV RNA from the liver (▪) and spleen (⊡) of VSV.LV-treated Balb/c mice. Quantitative RT-PCR (Q-RT-PCR) was used to measure GFP RNA. All samples were normalized to murine GAPDH. Values are presented as the means ± SEM for 3 mice per group. (B) Quantification of LV DNA from the liver (▪) and spleen (⊡) of VSV.LV-treated Balb/c mice. Spleen and liver samples were analyzed at the indicated time point by Q-PCR to measure LV copies per genome (C/G). All samples were normalized to murine β-actin. Values are presented as the means ± SEM for 3 mice per group. (C) Analysis of vector persistence in the liver. Q-PCR analysis was performed as in panel B on liver samples from mice treated with PBS (▴) or 10 μg of VSV.LV (▪) or bald.LV (•).