pDCs, but not CD11b⁺ splenocytes, are activated by LVs. (A) Phenotype analysis of isolated splenocyte populations. FACS acquisition of magnetic-sorted splenocytes was carried out to determine the phenotype of the isolated cells. Per analysis, 5 to 6 spleens were pooled (n=3). Representative plots are shown. (B) Cells from panel A were exposed to 600 ng/mL p24 of the indicated LV. After LV administration (5 days), FACS analysis was performed to measure GFP expression. (C) Supernatants from transduced cells were collected at 48 hours, and ELISA was used to measure IFNα and TNF-α concentrations. Values are presented as the means ± SEM (n=3).