PI3-kinase recruitment is required for CD28-mediated enhancement of T-cell adhesion and motility. (A) Murine T-cell hybridomas (5 × 105/transwell) expressing either human CD28 wild-type (top row) or M194CD28C were seeded onto M1, M1-CD80, and M1-CD86 monolayers grown on transwells. The average percentage of migrated cells after 24 hours in 3 independent experiments is shown. Standard error bars are shown. M1-CD80 versus M1, *P = .023; M1-CD86 versus M1, *P = .013. (B) CD28WT (top row) or M194CD28C (bottom row) T-cell hybridomas (indicated by arrows; 5 × 105/dish) were coincubated for 4 hours at 37°C in 25-mm Petri dishes with confluent M1, M1-CD80, or M1-CD86 cells. Images were analyzed by bright-field phase-contrast microscopy (scale bar: 20 μm).
