p53 family expression is dysregulated in rps19-deficient zebrafish. (A) Proteins of the p53 family have similar structure. They are expressed as full-length protein or truncated isoforms lacking all (Δ113p53) or most part of transactivation domain (ΔNp63 and ΔNp73). p63 and p73 can also be alternatively spliced at the C end. ΔNp63 and ΔNp73 can inhibit all full-length p53 family members. (B) Expression of p53 network genes in whole embryos received 3.4 ng of splicing blocking morpholino at different time points. RT-PCR, RNA was pooled from 30 embryos. W indicates wild-type; M, morphant embryos. (C) p53 is transcriptionally up-regulated in embryos injected with 3.4 ng of MOt morpholino inhibiting rps19 translation: RT-PCR. (D) p53 is up-regulated in red blood cell purified from 20 hpf embryos (pool of 300 embryos). ΔNp63 is not expressed in wild-type but induced in morphant cells. ADA is slightly up-regulated in morphant cells. Vertical lines have been inserted to indicate a repositioned gel lane.