American Society of Hematology

Figure 3

$Figure 3. Blast cell colony development from human EBs. (A) Photograph of a 6-day-old blast colony generated from day-3 EBs. Original magnification × 400. (B) Kinetics of blast colony development in EBs generated from H1 ES cells. Bars, where visible, indicate standard error of the mean number of colonies from 3 cultures. (C) Blast colony–forming potential of the presorted population (PS) and the KDR+CD117− (KDR), KDR−CD117+ (CD117), and KDR−CD117− (NEG) populations isolated from day-3 EBs. Top figure shows the gates used to isolate the different fractions. Bottom figure indicates the blast colony potential of each of the fractions as well as of the PS population. Bars indicating standard error of the mean number of colonies from 3 cultures are not visible. (D) Expression analyses of the different fractions isolated in panel C. (E) Expression analyses of 9 individual 6-day-old blast colonies generated from KDR+ cells isolated from day-4 EBs.$

Blast cell colony development from human EBs. (A) Photograph of a 6-day-old blast colony generated from day-3 EBs. Original magnification × 400. (B) Kinetics of blast colony development in EBs generated from H1 ES cells. Bars, where visible, indicate standard error of the mean number of colonies from 3 cultures. (C) Blast colony–forming potential of the presorted population (PS) and the KDR⁺CD117⁻ (KDR), KDR⁻CD117⁺ (CD117), and KDR⁻CD117⁻ (NEG) populations isolated from day-3 EBs. Top figure shows the gates used to isolate the different fractions. Bottom figure indicates the blast colony potential of each of the fractions as well as of the PS population. Bars indicating standard error of the mean number of colonies from 3 cultures are not visible. (D) Expression analyses of the different fractions isolated in panel C. (E) Expression analyses of 9 individual 6-day-old blast colonies generated from KDR⁺ cells isolated from day-4 EBs.

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