Detection of soluble HLA-E in sera from patients with AASV and correlation with soluble markers of inflammation. (A) Representative immunoblots showing the detection of sHLA-E in sera from patients with AASV (“Materials and methods”). The top panel shows the absence of sHLA-E in the serum from 3 healthy volunteers compared with negative and positive controls. The bottom panel illustrates the presence of sHLA-E in 3 sera from patients (nos. 1-3) with active phase and in 2 sera from 1 patient (no. 4). Rem indicates remission; Rel, relapse). Results are representative of 5 independent experiments. (B) Quantitative analysis of soluble IL-2Rα, CRP, IL-8, VEGF, and thrombomodulin by ELISA assays. *P < .01 versus normal; **P < .01 versus inactive. ND indicates not determined. Horizontal bars correspond to the means of value for each group of sera.