A c-met inhibitor (PHA665752) blunts the inhibitory effect of conditioned media from JJN cells on BMP-2–induced osteoblastogenesis. ALP activity of hMSCs treated with PHA665752 (100 nM), conditioned media from JJN3 cells diluted 1:10, and BMP-2 (300 ng/mL) for 4 days as indicated. Differences in ALP activity were statistically significant (independent samples, 2-tailed t test) in untreated cells versus cells treated with JJN3 Sup (P = .007), in untreated cells versus BMP-2–treated cells (P = .001), in BMP-2–treated cells versus cells treated with BMP-2/JJN-3 Sup (P = .008), and in BMP-2/JJN-3 Sup–treated cells versus cells treated with BMP-2/JJN-3 Sup/PHA665752.