Platelets bind adenovirus and express CAR on their surface. The 3H-labeled platelets were allowed to adhere on fibronectin-coated wells with or without the virus and the extent of adhesion was determined after lysis and measurement of radioactivity. (A) Graph showing reduced platelet adhesion in the presence of the virus compared to basal platelet adhesion (n = 4, P = .04) and significant interference with ADP potentiation of platelet adhesion (n = 4, P = .008). Error bars represent ± SEM. (B) Platelets and CAR-expressing HEK 293 cells were stained with mouse monoclonal anti–human CAR antibody and FITC rabbit anti–mouse IgG and analyzed with flow cytometry. The flow cytometry histogram shows that platelets are positive for CAR (graph representative of 4 experiments). (C) The presence of CAR on platelets was verified by human platelet mRNA analysis; 1.5% agarose gel showing RT-PCR of platelet-derived RNA amplifying 366-bp band from the CAR gene. Lane 1 is a 100-bp molecular weight ladder.
