Impaired IFNβ synthesis in LPS-stimulated neonatal blood. (A) Heparinized cord or adult blood was incubated with LPS (10 ng/mL) for the indicated time. mRNA was then extracted and real-time RT-PCR was performed. Results are expressed in mRNA copy numbers per 106 β-actin mRNA copies. The mean and SEM obtained from 5 different samples are shown. *P < .05 and **P < .01 cord versus adult blood. (B) Whole cord and adult blood samples were incubated with the indicated LPS concentration for 3 hours. The mean and SEM obtained from 5 different samples are shown. (C) Cord and adult hemocytes were washed and resuspended in either adult or neonatal plasma. Blood cells were stimulated for 24 hours in the presence of LPS (10 ng/mL). CXCL10 levels were analyzed by specific ELISA. Mean and SEM obtained from 10 different blood samples are shown. **P < .01 and ***P < .001.
