Impaired IFNβ synthesis and IFN-dependent gene expression in LPS-stimulated neonatal moDCs. Adult or cord blood moDCs were incubated with LPS (100 ng/mL) for the indicated times. Total RNA was extracted and real-time RT-PCR was performed for mRNA quantification. For determination of IFNβ levels in the supernatants, cells were stimulated for 24 hours. Histograms and error bars represent mean ± SEM. *P < .05, **P < .01, ***P < .001.