IFNβ signaling is functional in neonatal moDCs. (A-B) Stat-1 phosphorylation at Y701 in adult and cord blood moDCs was assessed by Western blotting. Cells were stimulated with LPS (100 ng/mL) (A) or recombinant IFNβ (10 U/mL) (B) for the indicated time. Total Stat-1 levels are shown as controls. One representative experiment of 3 is shown. (C) CXCL9 mRNA levels were determined in adult and cord blood moDCs by real-time RT-PCR. Cells were incubated with medium alone, LPS (100 ng/mL), and/or IFNβ (100 U/mL) for 6 hours. Mean and SEM from 5 different donors are shown. **P < .01 cord versus adult moDCs.