Blockade of CaMKII activation by KN62 protects mice from endotoxin shock after lethal LPS challenge. (A-C) Sex- and age-matched mice were injected intraperitoneally with 25-mg/kg doses of KN62 (the working concentration of KN62 is 7.2 g/L) (n = 8) or equal volume of dimethyl sulfoxide (n = 8) 30 minutes before intraperitoneal administration with 10 mg/kg body weight of LPS. Serum samples were obtained at 1.5 hours after LPS injection. Serum IL-6 (A), TNF-α (B), and IFN-β (C) were quantified by ELISA. Data represent mean plus or minus SD. *P < .05. Three experiments were performed with similar results. (D) Survival curve of mice (n = 8 per group) treated as described in panels A to C. The survival of the LPS-challenged mice was monitored for 7 days. *P < .05. Similar results were obtained in 3 independent experiments.