Multimer analysis of VWF from Y/Y1584 and Y/C1584 donors. (A) 1.4% (wt/vol) agarose gel. (B) 2.0% (wt/vol) agarose gel. Lanes 1 to 4 in each panel contain plasma VWF from 2 donors of each phenotype. (left) Multimer gel. (right) Densitometric scans of all lanes of the gel. A subtle but highly reproducible decrease in the upper satellite triplet band in heterozygous VWF was visible on the multimer gel and in the densitometric scans (arrows). This difference may extend to higher multimers but was beyond the resolution of the gel. The scale at the base of each scan corresponds to color intensity: 0 = black; 300 = white.