Analysis of expression of NF-κB/Rel subunits in primary ATLL cells by EMSA. Nuclear extracts from leukemic cells of (A) antiviral-sensitive (ATLL-1, ATLL-2, ATLL-3, ATLL-21, ATLL-27) and (B) antiviral-resistant (ATLL-4, ATLL-5, ATLL-6, ATLL-20, ATLL-22, ATLL-25) tumors were incubated with the indicated antibodies specific for the different NF-κB subunits and ³²P-labeled oligonucleotide containing a consensus NF-κB–binding sequence and were separated by gel electrophoresis. PBMCs from a healthy subject, isolated CD4⁺ cells from normal PBMCs, a leukemia patient with CD4⁺CD25⁺ mycosis fungoides/CTCL (HTLV-1 negative) (WBCs, 70 × 10⁹/L), and MT-2 cells (c-Rel⁺, HTLV-1⁺ line) were used as controls. The NF-κB complex was further characterized by supershifting with several antibody combinations in one patient (ATLL-6). WBC counts over time during induction AZT and IFN-α therapy curves are demonstrated in the selected leukemia patients.