Presence of active OCLs in DC-treated oc/oc mice. (A) Distribution of CFSE-labeled CD11c⁺ cDCs was analyzed by flow cytometry 48 hours after intraperitoneal injection into +/+ and oc/oc mice. (B) The absolute number of CFSE⁺CD11c⁺ DCs in the spleen and bone marrow (BM) of oc/oc mice was calculated by multiplication of the percentage of CFSE⁺CD11c⁺ DCs by the total number of resident leukocytes. Data are the mean plus or minus SD of the number of recovered CFSE⁺CD11c⁺ DCs per 10⁶ injected cells (n = 4 to 6 per group) and are representative of 2 independent experiments. (C) Representative histologic analysis of the femora of +/+ mice, control, and DC-injected oc/oc mice. TRAP⁺ cells were detected in all mice, but a3-expressing cells were detected in +/+ and DC-treated oc/oc mice but not in control oc/oc mice. (D) Bone marrow cells from +/+ mice, control, and DC-treated oc/oc mice were cultured onto dentine slices, and their bone resorption activity was analyzed. Resorption lacunae (arrows) were detected in +/+ and DC-treated oc/oc mice but not in control oc/oc mice. Images were captured using an Axioskop microscope (Carl Zeiss) with a 20×/0.75 Fluar objective (Carl Zeiss), an AxioCam HRc camera (Carl Zeiss), and AxioVision 4.5 software (Zeiss).