Figure 1
![Figure 1. DNA synthesis and mRNA levels in HUVECs. HUVECs were treated with 50 μM DL-Hcy and 1 mM AZC in control medium for 48 hours, or 300 nM TSA and 5 mM NaBr for 24 hours. (A) Thymidine uptake. Cells were metabolically labeled with 1 μCi (0.037 MBq)/mL [3H]-thymidine during the last 3 hours. Values represent mean (± SD) from 3 separate experiments with triplicates (n = 9). *P < .01 versus non-Hcy and nonviral control. #P < .01 versus Hcy-treated and non–viral-transduced group. (B) Northern blot analysis. Total cellular RNA was used for Northern analysis (10 μg). The blot was hybridized with the probes of human cyclins A and D1, and subsequently with 18S RNA.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/110/10/10.1182_blood-2007-06-096701/6/zh80230709760001.jpeg?Expires=1735580855&Signature=11feGvxhLoXfSKxBZz112MLzGels8pAv6dwU4Za9Lc1BII2S59zHe4LptB8Kdv8WxyfHZwgViEfNSseiAzxBn3vtoBxxca8nK1RBb2U6hVUxA7P9d27XYG34oixeFqDxojBln5tQ7CNhHCf2t7~SEGhypMVpSzLu03WVU1IH6V3l-7Y5Dg1d7JYozxaZif6h8smlMQSn0io9XMk6kFZiQPLE3f53PP99ia0TfwwXS1aWuO9gd2YM9i5egNpI1ElyBbgtU4FGVb7J-yQLkIddxaxJNCTQjbD~JHMzzPdPcEkB5dzOeIJIkwaSv8tYyE1JPYEsHynaFgnYqg~zjmEUqQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
DNA synthesis and mRNA levels in HUVECs. HUVECs were treated with 50 μM DL-Hcy and 1 mM AZC in control medium for 48 hours, or 300 nM TSA and 5 mM NaBr for 24 hours. (A) Thymidine uptake. Cells were metabolically labeled with 1 μCi (0.037 MBq)/mL [3H]-thymidine during the last 3 hours. Values represent mean (± SD) from 3 separate experiments with triplicates (n = 9). *P < .01 versus non-Hcy and nonviral control. #P < .01 versus Hcy-treated and non–viral-transduced group. (B) Northern blot analysis. Total cellular RNA was used for Northern analysis (10 μg). The blot was hybridized with the probes of human cyclins A and D1, and subsequently with 18S RNA.
