Figure 4
![Figure 4. DNMT activities and protein levels. HUVECs were treated with 50 μM DL-Hcy or 1 mM AZC in control medium for 48 hours. Nuclear extracts (20 μg) were prepared and incubated with hemimethylated double-stranded DNA for DNMT1 activity (A) or with unmethylated double-stranded DNA for DNMT3 activity (B) in the presence of S-adenosyl-L-[methyl-3H] methionine. DNMT activities were determined by the radioactivity level of DNA substrates. Values represent mean (± SD) from 3 separate experiments with triplicated samples (n = 9). *P < .01 versus control. Protein levels of DNMT1 and DNMT3 were examined by Western blotting with antibodies against DNMT1 or DNMT3, respectively, and reblotted with β-actin antibody.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/110/10/10.1182_blood-2007-06-096701/6/zh80230709760004.jpeg?Expires=1735581611&Signature=pvFBqrkqQT5LHeidF1ZNMsJ2kkx41vZSXvtoXi1-657V-2pYP1bi8d37NwE9rSH8M4XU79wrtV2~UhfJ~AA25tqFCPqFVS7v7IvYS0VPkWjXalaNdnRPrUAl8Fbxe6P92t1Qp8B8OvfZWt6r1McnpzjXywQzz~Cccr9DSFbr5zjdwJ8vugarHAmB-kxqgroz8a0FihSCKjgjrg0FFrC25ydlk91sCu5m3~o1-uwELKspN6GNtqv1rhbwIOYrsVzITfkZm7fTI9EjWzos2C0OODPUVjTDy70Fq988Gy8-uyRf7TEXl0a7HWmT7GN69nen7V8~mb1BB8vVIlDqsMnZdA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
DNMT activities and protein levels. HUVECs were treated with 50 μM DL-Hcy or 1 mM AZC in control medium for 48 hours. Nuclear extracts (20 μg) were prepared and incubated with hemimethylated double-stranded DNA for DNMT1 activity (A) or with unmethylated double-stranded DNA for DNMT3 activity (B) in the presence of S-adenosyl-L-[methyl-3H] methionine. DNMT activities were determined by the radioactivity level of DNA substrates. Values represent mean (± SD) from 3 separate experiments with triplicated samples (n = 9). *P < .01 versus control. Protein levels of DNMT1 and DNMT3 were examined by Western blotting with antibodies against DNMT1 or DNMT3, respectively, and reblotted with β-actin antibody.
