Chromatin remodeling (CHIP assay). HUVECs and HASMCs were treated with 50 μM DL-Hcy and 1 mM AZC in control medium for 48 hours, or 300 nM TSA and 5 mM NaBr for 24 hours. Nuclear extracts were immunoprecipitated with antibody against MeCP2 (A,B), and AcH3 or AcH4 (C). PCR was performed using primers specific for cyclin A promoter from position −267 to 37. Input DNA was amplified to evaluate the total amount of DNA applied to the immunoprecipitation.