Normal bone marrow hematopoiesis but increased turnover of myeloid monocytic precursors in aged Aire^–/– mice. (A) Differential count of monocytes and lymphocytes on blood smears. n = 7 WT, 7 KO. (B) Mean fluorescence intensity of CD11b in CD11b^hi cells in bone marrow measured by flow cytometry (left panel); n = 8 WT, 8 KO. Each symbol corresponds to a different animal. P value was calculated with t test. Mean values are indicated by horizontal lines. Size and granularity of CD11b^hiGr1^lo/–IAb^– bone marrow myeloid cells (right panel); gating strategy shown in the middle panel. n = 6 WT, 5 KO. Two representative mice are shown. (C) One-hour BrdU incorporation in CD11b^hi IAb^– and CD11b^hi IAb⁺ bone marrow myeloid cells gated on monocyte precursors from FSC/SSC. n = 6 WT, 5 KO. Two representative mice are shown.