![Figure 1. Neutrophil differentiation induces TG2 expression both in the cytosol and the nucleus of NB4 cells. (A) Transglutaminase activity in cells lysates. Cells were treated with 1 μM ATRA for 7 days during which TG2 enzymatic activity was measured in their cytosolic and nuclear fractions. Activity was measured by detecting incorporation of [3H]putrescine into casein. The amount of incorporated [3H]putrescine was determined in a beta-counter. Bars depict the means of 3 separate experiments each performed in duplicate. Error bars indicate standard deviation (SD). (B) Western blot analysis of TG2 in NB4 cells. Cytosolic and nuclear fractions were separated by SDS-PAGE. Total protein (25 μg) was loaded in each lane, and blots were developed with CUB7402 monoclonal antibody against TG2.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/108/6/10.1182_blood-2004-02-007948/4/zh80180600970001.jpeg?Expires=1735849960&Signature=ztLwWkEYq5TNgzPQx8zhv7E0O3qLtXz73XH3cTFI~h0pCVOaRvKf9Z9hvWRAlLtFo1n~eSrsw3mrn79WvlZxh92r5fnhROKTOYUTDY3054ulzOjVdzXVxecwuHJlbfnNJM1RviGiNWc79DSuAG0~ksIOODLOaLrq48Q287Ete48lmcBGaZ63R9AqELFurIF-l6e4UvvljobJeUg1nAcGZn1YemZBT1hneAi3qx11XaateagdxoTGb77c4OxuKGCRU6A1Ii4kCEhvZzzMjDRSOwJLyXu2whKVanZwateW5w226keATGX0pAxrEplakyJ4uwPty5psNUfSPGfeTFZ6BQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Neutrophil differentiation induces TG2 expression both in the cytosol and the nucleus of NB4 cells. (A) Transglutaminase activity in cells lysates. Cells were treated with 1 μM ATRA for 7 days during which TG2 enzymatic activity was measured in their cytosolic and nuclear fractions. Activity was measured by detecting incorporation of [3H]putrescine into casein. The amount of incorporated [3H]putrescine was determined in a beta-counter. Bars depict the means of 3 separate experiments each performed in duplicate. Error bars indicate standard deviation (SD). (B) Western blot analysis of TG2 in NB4 cells. Cytosolic and nuclear fractions were separated by SDS-PAGE. Total protein (25 μg) was loaded in each lane, and blots were developed with CUB7402 monoclonal antibody against TG2.
