Hematopoietic chimerism does not impair immune response to third-party antigens. (A) Chimeric or naive female mice were challenged subcutaneously with 100 μg OVA protein emulsified in IFA. Splenocytes were tested at day 10 in a standard IFNγ ELISPOT assay against the OVA257 epitope. Serum was tested by ELISA for anti-OVA antibody. Results represent the mean of 3 mice per group ± SEM. (B-C) Susceptibility of male cells to immune cytolytic activity. Chimeric mice were challenged subcutaneously with OVA protein in IFA as in panel A and were infused at day 8 with male splenocytes (n = 2) or female splenocytes (n = 2) either pulsed with OVA257 (0.5 μM CFSE) or left unpulsed (5 μM CFSE). PBMCs were analyzed at day 0, 1, and 2. The percentage of specific lysis of pulsed over unpulsed cells was calculated as detailed in “Materials and methods,” similarly to Figure 5 with male over female cells. Panels B and C represent 1 of 2 similar results.