Delayed kinetics of clot retraction for TSSC6–/– platelets in the presence of normal integrin αIIbβ3 expression. (A) Photographs showing in vitro kinetics of clot retraction over a 21-hour time frame using platelet rich plasma (PRP) (normalized platelet counts) from wild-type and TSSC6-deficient mice. Samples were treated with 2.5 units thrombin. Each photograph is representative of at least 3 experiments. (B) The expression of surface markers on platelets was determined by staining with an isotype control (FITC-CD3), positive control FITC-CD44 mAb, FITC-CD9, and FITC-integrin β3 mAb for both wild-type and TSSC6–/– platelets. FITC-labeled samples were analyzed on a FACS Calibur analyzer. Results are cumulative data derived from 3 independent experiments and presented as MFI ± SEM.
