TSSC6^–/– platelets display restricted cytoskeletal reorganization upon spreading on immobilized fibrinogen. (A) Washed wild-type and TSSC6^–/– platelets were allowed to adhere for 0 to 40 minutes at 37°C to a fibrinogen matrix. Adherent platelets were fixed and imaged by DIC microscopy. Images were captured with an Axiovert 135 microscope (Zeiss, Oberkochen, Germany) with a 63×/1.25 oil immersion lens and a PixeLINK megapixel firewire camera (model PL-A661; PixeLINK, Ottawa, ON, Canada) and PixeLINK software version 3.2. (B) The percentage of spread platelets for each genotype were quantitated per high-powered field (*P < .05, n = 3). DIC images shown are a representative of 3 independent experiments. (C) The number of adherent platelets for each genotype was quantitated per high-powered field (P > .05; n = 3). Results are representative of 3 independent experiments.