American Society of Hematology

Figure 5.

$Figure 5. TGF-β1 up-regulates and then down-regulates the GTP-bound form of RhoGTPases. (A) Cells were incubated with or without 5 ng/mL TGF-β1 for the indicated times at 37°C and lysed in a buffer containing 1% Triton X-100. GST-RBD and GST-PBD were mixed with the cell extracts, which were incubated with GSH-Sepharose beads. The beads were washed, and bound proteins were fractionated on SDS-PAGE. Western blotting was performed with anti-RhoA, anti-Rac1, and anti-Cdc42 antibodies. (B) Protein amounts expressed by Western blot were quantitated by densitometry, and relative amounts of GTP-bound form at the indicated time were presented as fold. GTP-bound form/total protein at time 0 was expressed as 1. The values were means ± SE of 3 independent experiments (*P < .05).$

TGF-β1 up-regulates and then down-regulates the GTP-bound form of RhoGTPases. (A) Cells were incubated with or without 5 ng/mL TGF-β1 for the indicated times at 37°C and lysed in a buffer containing 1% Triton X-100. GST-RBD and GST-PBD were mixed with the cell extracts, which were incubated with GSH-Sepharose beads. The beads were washed, and bound proteins were fractionated on SDS-PAGE. Western blotting was performed with anti-RhoA, anti-Rac1, and anti-Cdc42 antibodies. (B) Protein amounts expressed by Western blot were quantitated by densitometry, and relative amounts of GTP-bound form at the indicated time were presented as fold. GTP-bound form/total protein at time 0 was expressed as 1. The values were means ± SE of 3 independent experiments (*P < .05).

This Feature Is Available To Subscribers Only