Synthesis of variant fibrinogens in transfected CHO cells. The concentrations of fibrinogen in the culture media (A) and cell lysates (B) were measured by ELISA as described in “Materials and methods.” Fibrinogen concentration ratios of medium to cell lysate are shown in panel C. The mean values are presented with standard deviations indicated by the error bars. Concentrations were determined for 8 to 9 isolates of the CHO lines expressing γ387I (I), γ387R (R), γ387L (L), γ387M (M), γ387A (A), and γ387D (D). Significantly different from γ387I (*P < .05, **P < .01, ***P < .001).