Reduction in the number of immunophenotypically defined GMPs from GATA-2^+/− marrow. Bone marrow–nucleated cells from each genotype were lineage depleted using magnetic bead separation and lineage-negative–enriched cells were stained with various cell-surface antibodies to enable isolation of immunophenotypically defined progenitor subsets by flow cytometry. Representative flow cytometry plots for (Lin/CD127⁻CD117⁺Sca-1⁻CD34⁺CD16/32^high) GMP, (Lin/CD127⁻CD117⁺Sca-1⁻CD34⁺CD16/32^lo) CMP, and (Lin/CD127⁻CD117⁺Sca-1⁻CD34⁻CD16/32⁻) MEP isolation are shown (A) and for (Lin⁻CD117⁺Sca-1⁺Flt-3⁺CD34⁺) LMPP (B). The percentages for each gate are shown. The absolute number of LMPP, CMP, GMP, and MEP progenitors produced per harvest from multiple experiments is depicted (C) (LMPP, n = 4, P = .38; CMP, n = 9, P = .15; GMP, n = 9, P < .001<; and MEP, n = 9, P = .1). ** indicates statistical significance. Error bars indicate SEM. Statistical analysis was performed by using the paired Student t test.