PDC supernatant inhibits replication of both CXCR4- and CCR5-using HIV-1. (A) The PM1 T-cell line was infected with CXCR4-using LAI (filled markers) or CCR5-using JR-CSF (open markers) in the presence (triangles) or absence (diamonds) of supernatant from SAC-stimulated PDCs. HIV-1 replication was followed by measuring CA-p24 accumulation in the supernatant with ELISA. (B, C) PM1 T cells (B) or LuSIV cells (C) were infected with CXCR4-using HIV-1 LAI in the presence of PDCs, or PDC supernatant dilutions. RLU, relative light units. Error bars represent standard deviations. *P < .01 compared with medium control.