American Society of Hematology

Figure 6.

$Figure 6. PDCs secrete an additional HIV-1 inhibitory factor of less than 3 kDa. (A) PDC supernatant was size fractionated by filter centrifugation into fractions of less than 3 kDa, 3 to 10 kDa, 10 to 30 kDa, 30 to 100 kDa, and more than 100 kDa. These fractions were incubated with LuSIV cells and HIV-1 for 24 hours, followed by luciferase measurement (▪). All fractions were also tested for the presence of IFNα by ELISA (▦). *P < .001 compared with control (ie, HIV-1 only). (B) LuSIV cells and the less than 3 kDa and 30 to 100 kDa fractions were preincubated with type I IFN blocking antibodies, followed by coculture and HIV-1 infection. Luciferase was measured 24 hours later. *P < .01 compared with controls with mock supernatant. #P < .01. NS, not significant; RLU, relative light units. Error bars represent standard deviations.$

PDCs secrete an additional HIV-1 inhibitory factor of less than 3 kDa. (A) PDC supernatant was size fractionated by filter centrifugation into fractions of less than 3 kDa, 3 to 10 kDa, 10 to 30 kDa, 30 to 100 kDa, and more than 100 kDa. These fractions were incubated with LuSIV cells and HIV-1 for 24 hours, followed by luciferase measurement (▪). All fractions were also tested for the presence of IFNα by ELISA (▦). *P < .001 compared with control (ie, HIV-1 only). (B) LuSIV cells and the less than 3 kDa and 30 to 100 kDa fractions were preincubated with type I IFN blocking antibodies, followed by coculture and HIV-1 infection. Luciferase was measured 24 hours later. *P < .01 compared with controls with mock supernatant. #P < .01. NS, not significant; RLU, relative light units. Error bars represent standard deviations.

This Feature Is Available To Subscribers Only