Detection of MPL 1544 G>T and MPL 1543 TG>AA (MPL W515) mutations in peripheral blood cells from PMF patients. Peripheral cells from MPL-mutated PMF patients were isolated using standard density, immunomagnetic and flow cytometric methods for further DNA extraction, and sequence analysis. The MPL 1544 G>T and MPL 1543 TG>AA mutations were detected in some CD34⁺ cells, granulocytes, and platelets cells from 2 of the PMF patients. The CD34⁺/38⁻ cells, CD34⁺/38⁺ cells, granulocytes, platelets, monocytes, NK cells, and T- and B-cell sequence traces from 3 PMF patients are shown. Red arrows indicate the presence of a mutant peak.