Long-term reconstitution in NOD/SCID mice with CD34⁺CD38⁻ cells from MPL W515 PMF. Human MPL mutant PMF CD34 cells (5-6.6 × 10⁵) were injected into NOD/SCID mice or harvested in methylcellulose assay to determine the frequency of 100% mutated (■), 50% mutated (), or unmutated (□) human myeloid colonies before or 3 and 6 weeks after transplantation. In parallel, chimerism of human cells was determined using CD45 expression by flow cytometry. Twelve weeks after transplantation, chimerism was too low to perform methyl assay but SNP analysis was performed. Results from 2 different MPL mutant reconstituted mice are shown.