Reduced number of splenic CFUe's after hemolytic stress. (A) Single-cell suspensions from BM of control and β1β7 mutant mice (no BM transplantation) untreated and treated at day 1 and 2 with PHZ were prepared at day 4, stained with Ter119 antibody, and subsequently analyzed by FACS. The total amount of Ter119⁺ erythroblasts per femur is shown. Error bars show the standard deviation (n (controls)/(β1β7 mutants): 4/4). (B) BM single-cell suspensions from PHZ treated β1β7 mutant BM chimeras were prepared, stained with antibodies against α4, β1 integrin, CD71, and Ter119 and subsequently analyzed by FACS. (Ter119-CD71 staining distinguishes 5 different developmental stages.) (C) The amount of erythroid BM cells of PHZ-treated β1β7 mutant mice for each of the 5 developmental stages as distinguished by Ter119 and CD71 is shown. Error bars show the standard error (n (controls)/(β1β7 mutants): 4/4). (D) Single-cell suspensions from BM of control and β1β7 mutant mice (no BM transplantation) treated at day 1 and 2 with PHZ were prepared at day 4 and tested for CFUe's. Total numbers of colonies per femur and spleen are shown. Error bar shows the standard error (n (controls)/(β1β7 mutants): 3/3).