K5 inhibits CD31 expression. (A) E-DMVECs infected with AdK5, AdK3, AdMK3, or AdTet control were analyzed for CD31 or MHC I expression by flow cytometry. The shaded graph represents AdTet-infected cells, whereas the unshaded graph represents cells transduced with the indicated adenovirus constructs. (B) At 24 hours after transduction with indicated viruses, E-DMVECs were stained with fluorescently labeled FLAG antibody to detect MK3, K5, or K3 (left panel; green) and costained for CD31 (middle panel, red) or MHC I (right panel; red).