K5 inhibits migration of endothelial cells. HUVECs were transduced with AdEGFP or AdK5 as described in “Materials and methods.” The monolayers were scratched using a pipet tip and the resealing of the wounds was monitored over the indicated time points by phase microscopy. (A) Photographs were taken at regular intervals of the identical region of a representative part of each wound. (B) The width of each wound was measured and plotted as the percentage of the initial wound width versus time. The average of 3 independent experiments is shown.