Direct interactions between FANCL and FANCB in the mammalian 2-hybrid assay. Protein pairs were cotransfected in 293 cells in the presence of a luciferase reporter construct to test for direct interactions. All experiments were performed in triplicate. (A) AD-FANCL was cotransfected with BD-FA proteins. Fold induction of luciferase expression is relative to FANCL alone. (B) AD-FANCB was cotransfected with BD-FA proteins. Fold induction of luciferase expression is relative to FANCB alone.