Effect of dominant-negative mutants on long-term repopulating ability of LDBM cells. (A) Evolution of chimerism in the peripheral blood of transplant recipient animals. Data represent percentage change in the peripheral-blood chimerism compared with the control group beginning 3 weeks after transplantation; MIEG3 (♦, control group), RhoAN19 (▪), Rac2D57N (▴). *P < .05 versus control group. The data represent the mean ± SEM of 3 independent experiments (n = 11). See initial engraftment raw data for each independent experiment in Table S1 (available on the Blood website; see the Supplemental Materials link at the top of the online article). (B) Lineage analysis of peripheral blood of the recipient animals (shown in panel A), 16 weeks after transplantation; CD3e (▪), B220/CR45R (dark gray bars), Gr1 (light gray bars), other (□). The data are normalized to the level of engraftment in each individual animal and represent the mean ± SEM of 3 independent experiments, *P < .01. See lineage analysis directly related with the proportion of EGFP⁺ cells from a representative experiment in Figure S1. (C) Analysis of transduction of progenitor (CFU) cells before and after serial transplants; control (▪), RhoAN19 (dark gray bars), and Rac2D57N (light gray bars). Data are represented as percentage change relative to control group before transplantation and represent the mean ± SEM of 3 independent experiments (N = 11), *P < .05 versus control group.