Ag uptake and surface expression of MHC class II and costimulatory molecules are reduced in C3^−/− DCs and C3aR^−/− DCs. (A) Ag uptake in 6-day DCs prepared from C3^−/− mice and WT mice. The data are shown as mean of triplicate samples. A representative of 5 independent experiments is shown. (B) Ag uptake in 6-day DCs prepared from C3aR^−/− mice and WT mice. Data are shown as mean of triplicate samples. A representative of 3 independent experiments is shown. (C-E) Surface expression of MHC-II, CD40, and B7.2 in 7-day DCs by flow cytometry. (C) Representative histogram plots from WT DCs, C3^−/− DCs, and C3aR^−/− DCs. (D,E) Six-day DCs were incubated with or without OVA (250 μg/mL, 40 minutes) and washed with cold PBS. The washed DCs were further cultured for 24 hours and then used for the analysis. (D) C3^−/− DCs versus WT DCs. (E) C3aR^−/− DCs versus WT DCs. The data are shown as mean of duplicate samples. A representative of 3 independent experiments is shown.