Differential effects of C3aR on phosphorylation pathways of signal transduction. (A-C) Detection of AKT (PI3K), ERK1/2, and P38 (MAPK) phosphorylation in 6-day DCs (105 cells) after C3a stimulation, by Western blot analysis. In each blot the top row of bands corresponds to incubating membrane with appropriate total antibody and the bottom row of bands corresponds to incubating membrane with appropriate antiphospho antibody. A representative of 3 independent experiments is shown. (D,E) Effect of inhibition of AKT, ERK, and P38 pathways on Ag uptake and Ag presentation in DCs. Five-day DCs were further cultured for 24 hours in the presence or absence of the appropriate inhibitor (ie, wortmanin for AKT; U0126 for ERK; SB202190 for P38) and then were used for assays for Ag uptake and Ag presentation. Data are shown as mean plus or minus SEM (n = 4 for Ag uptake; n = 8 for proliferation). A representative of 2 independent experiments is shown.
