Maml genes are differentially expressed in hematopoietic and lymphoid progenitor subsets. Quantitative RT-PCR was performed on cDNA isolated from multipotent BM progenitors, thymocyte subsets, BM B lineage progenitors, and splenic B-cell subsets from C57BL/6 mice fractioned by flow cytometry. Maml1-Maml3 transcript levels were normalized using Hprt expression. The Maml1/Hprt ratio in LSK progenitors was normalized to 1. The measurements were performed in triplicates. Results are shown as means plus or minus SD. (A) Relative expression of Maml1-Maml3 in multipotent BM progenitors (LSK subset) and thymocyte subsets. LSK indicates Lin⁻Sca-1⁺c-kit⁺; ETP, early T lineage progenitors (Lin⁻c-Kit⁺CD25⁻); DN2, CD4⁻CD8⁻ double-negative 2 (Lin⁻c-Kit⁺CD25⁺); DN3, double-negative 3 (Lin⁻c-Kit⁺CD25⁻); and DP, CD4⁺CD8⁺ double-positive thymocytes. (B) Maml expression in BM B-cell progenitor subsets. CLP indicates common lymphoid progenitors (Lin⁻Sca-1^loc-kit^loIL-7Rα⁺Flt3⁺); ProB, pro-B cells (B220⁺CD43⁺AA4.1⁺CD19⁺sIgM⁻); PreB, pre-B cells (B220⁺CD43⁻AA4.1⁺sIgM⁻); and ImmB, immature B cells (B220⁺CD43⁻AA4.1⁺sIgM⁺). (C) Maml expression in splenic B-cell subsets. Tr1-Tr3 indicates successive stages of immature transitional B cells (Tr1, B220⁺AA4.1⁺sIgM^hiCD23⁻; Tr2, B220⁺AA4.1⁺sIgM^hiCD23⁺; Tr3, B220⁺AA4.1⁺sIgM^intCD23⁺); FoB, follicular B cells (most abundant mature B-cell type in the spleen; B220⁺AA4⁻sIgM^intCD23⁺); MZP B cells, marginal zone B cell precursors (B220⁺AA4.1⁻sIgM^hiCD21^hiCD23⁺); MZB, marginal zone B cells (B220⁺AA4.1⁻sIgM^hiCD21^hiCD23^lo).